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caspase 3 7 biosensor  (Sartorius AG)


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    Sartorius AG caspase 3 7 biosensor
    Caspase 3 7 Biosensor, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 98/100, based on 1107 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/caspase+3+7+biosensor/Incucyte+Caspase-3+7+Dye+for+Apoptosis/pm35696676-145-21-28
    Average 98 stars, based on 1107 article reviews
    caspase 3 7 biosensor - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Activity Assay:

    Article Title: Multiparametric High-Content Cell Painting Identifies Copper Ionophores as Selective Modulators of Esophageal Cancer Phenotypes
    Article Snippet: For the expanded panel of EAC cell lines, data CellTitre-Glo (promega) was added according to the manufacturer’s instructions and incubated for 10 min at room temperature before being read. .. For caspase-dependent apoptosis assays, we monitored caspase activity at sequential time points following compound treatments using the Incucyte imaging instrument and caspase-3/7 biosensor according to the manufacturer’s instructions (Sartorius). .. CellProfiler v3.1.5 software was used to extract features from the images.

    Article Title: Multiparametric High-Content Cell Painting Identifies Copper Ionophores as Selective Modulators of Esophageal Cancer Phenotypes.
    Article Snippet: For the expanded panel of EAC cell lines, data CellTitre-Glo (promega) was added according to the manufacturer’s instructions and incubated for 10 min at room temperature before being read. .. For caspase-dependent apoptosis assays, we monitored caspase activity at sequential time points following compound treatments using the Incucyte imaging instrument and caspase-3/7 biosensor according to the manufacturer’s instructions (Sartorius). ..

    Imaging:

    Article Title: Multiparametric High-Content Cell Painting Identifies Copper Ionophores as Selective Modulators of Esophageal Cancer Phenotypes
    Article Snippet: For the expanded panel of EAC cell lines, data CellTitre-Glo (promega) was added according to the manufacturer’s instructions and incubated for 10 min at room temperature before being read. .. For caspase-dependent apoptosis assays, we monitored caspase activity at sequential time points following compound treatments using the Incucyte imaging instrument and caspase-3/7 biosensor according to the manufacturer’s instructions (Sartorius). .. CellProfiler v3.1.5 software was used to extract features from the images.

    Article Title: Multiparametric High-Content Cell Painting Identifies Copper Ionophores as Selective Modulators of Esophageal Cancer Phenotypes.
    Article Snippet: For the expanded panel of EAC cell lines, data CellTitre-Glo (promega) was added according to the manufacturer’s instructions and incubated for 10 min at room temperature before being read. .. For caspase-dependent apoptosis assays, we monitored caspase activity at sequential time points following compound treatments using the Incucyte imaging instrument and caspase-3/7 biosensor according to the manufacturer’s instructions (Sartorius). ..



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    Image Search Results


    Schematic diagram showing the mechanistic basis for the GloSensor Caspase 3/7 reporter. In response to apoptosis, activation of Caspase 3/7 leads to cleavage of the intervening peptide DEVD in the GloSensor Caspase 3/7 reporter. This caspase-mediated cleavage of the DEVD sequence results in the reconstitution of the enzymatic activity and a resultant increase in bioluminescence in the presence of luciferin substrate (shown in purple)

    Journal: Proteases and Cancer

    Article Title: Imaging Proteolytic Activities in Mouse Models of Cancer

    doi: 10.1007/978-1-4939-7595-2_22

    Figure Lengend Snippet: Schematic diagram showing the mechanistic basis for the GloSensor Caspase 3/7 reporter. In response to apoptosis, activation of Caspase 3/7 leads to cleavage of the intervening peptide DEVD in the GloSensor Caspase 3/7 reporter. This caspase-mediated cleavage of the DEVD sequence results in the reconstitution of the enzymatic activity and a resultant increase in bioluminescence in the presence of luciferin substrate (shown in purple)

    Article Snippet: The GloSensor Caspase 3/7 biosensor is encoded by the pGLS-30F construct and is also commercially available (GLS Caspase 3/7, Promega).

    Techniques: Activation Assay, Sequencing, Activity Assay

    Schematic representation of the GloSensor Caspase 3/7 reporter . The reporter comprises of an N-terminal domain coding for the C terminus (358–544) of luciferase and C-terminal domain coding for the N terminus (4–354) of firefly luciferase with an intervening Caspase 3/7 recognition sequence DEVD

    Journal: Proteases and Cancer

    Article Title: Imaging Proteolytic Activities in Mouse Models of Cancer

    doi: 10.1007/978-1-4939-7595-2_22

    Figure Lengend Snippet: Schematic representation of the GloSensor Caspase 3/7 reporter . The reporter comprises of an N-terminal domain coding for the C terminus (358–544) of luciferase and C-terminal domain coding for the N terminus (4–354) of firefly luciferase with an intervening Caspase 3/7 recognition sequence DEVD

    Article Snippet: The GloSensor Caspase 3/7 biosensor is encoded by the pGLS-30F construct and is also commercially available (GLS Caspase 3/7, Promega).

    Techniques: Luciferase, Sequencing

    Development of transgenic mice conditional for the Caspase 3/7 GloSensor reporter. A genetically engineered transgenic mouse was generated using an expression cassette wherein the GloSensor Caspase 3/7 reporter was preceded by a floxable GFP/stop cassette in pCLEX vector driven by CAG promoter. Reporter expression is achieved in a Cre-dependent manner which can simultaneously be detected by the loss of EGFP expression

    Journal: Proteases and Cancer

    Article Title: Imaging Proteolytic Activities in Mouse Models of Cancer

    doi: 10.1007/978-1-4939-7595-2_22

    Figure Lengend Snippet: Development of transgenic mice conditional for the Caspase 3/7 GloSensor reporter. A genetically engineered transgenic mouse was generated using an expression cassette wherein the GloSensor Caspase 3/7 reporter was preceded by a floxable GFP/stop cassette in pCLEX vector driven by CAG promoter. Reporter expression is achieved in a Cre-dependent manner which can simultaneously be detected by the loss of EGFP expression

    Article Snippet: The GloSensor Caspase 3/7 biosensor is encoded by the pGLS-30F construct and is also commercially available (GLS Caspase 3/7, Promega).

    Techniques: Transgenic Assay, Generated, Expressing, Plasmid Preparation